CLM2 — Magnaporthe oryzae 70-15

Summary

MoCLM2 (MGG_04432) on chromosome 2 of Magnaporthe oryzae 70-15 encodes a cytochrome P450 family protein with sterol 14-demethylase activity, monooxygenase activity, heme and iron binding, and methyltransferase activity. The gene is associated with ergosterol and sterol biosynthesis, demethylation, mycotoxin production, sporulation, hyphal growth, and mitophagy. It localizes to the endoplasmic reticulum membrane and membrane. Phenotypic associations link MoCLM2 to decreased micronucleophagy, nucleophagy, and ribophagy; abnormal cell cycle progression, G0 entry, and cytokinesis; increased cell death; arrested vegetative and exponential growth; decreased invasive growth, nutrient uptake, and iron and phosphorus utilization; and increased fermentative metabolism and lifespan. The gene is also connected to rice blast disease. Studies of related genes in other species link the family to sterol 14α-demethylase activity and loss of pathogenicity.