RAD18 — Magnaporthe oryzae 70-15

Summary

MGG_04175 (MoRAD18) encodes a RING-type E3 ubiquitin transferase RAD18, a member of the Rad18_fungi family containing SAP and zinc-finger RING domains. The enzyme exhibits ssDNA-dependent ATPase activity, ubiquitin ligase activity, and ssDNA binding. MoRAD18 is associated with DNA damage tolerance and repair, including error-free and mutagenic translesion synthesis, interstrand crosslink repair, and mitotic recombination. It is linked to protein monoubiquitination, appressorium formation, sporulation, and methylation. The protein localizes to chromatin and the nucleus. Phenotypic associations include altered cell cycle progression (G1/S and S phase), increased transposable element transposition, abnormal mitotic and meiotic recombination, delayed sporulation, and altered resistance to chemicals. Studies of related genes in other species link them to hypervirulence and the oxidative stress response.