DOT — Fusarium graminearum PH-1

Summary

FGRAMPH1_01G01733 (DOT, also known as SAC1) in Fusarium graminearum PH-1 encodes a SAC domain-containing protein with phosphatidylinositol-4-phosphate phosphatase activity and multiple inositol polyphosphate 5-phosphatase activities, as well as calcium ion binding. The gene is associated with phosphoinositide dephosphorylation, sphingosine anabolism, cell wall biogenesis, protein transport, and signal transduction. It localizes to the Golgi membrane, endoplasmic reticulum membrane, mitochondrial outer membrane, fungal-type vacuole membrane, and the cell cortex and division site. Phenotypic associations include abnormal spore germination, decreased budding, absent cell cycle progression, increased endocytosis, abnormal vacuolar and Golgi distribution, absent hyperosmotic stress resistance, and absent innate thermotolerance. Studies of related genes in other species link the family to vacuolar transport, endocytosis, septum formation, and utilization of iron and phosphorus sources.