KGD1 — Verticillium dahliae

Summary

VDAG_10018, designated VdKGD1, encodes a protein with 2-oxoglutarate dehydrogenase (lipoamide) activity (EC 1.2.4.2) that binds TPP and metal ions, and contains domains including 2-oxoglutarate dehydrogenase E1. The gene is associated with the TCA cycle, 2-oxoglutarate metabolism, heterotrophy, and response to oxidative stress, and its product localises to mitochondria and the mitochondrial nucleoid. VdKGD1 has been linked to vascular wilt disease in cotton (Gossypium hirsutum) and to leaf chlorosis. Phenotypic associations include abnormal colony colour and size, increased cell death, altered mitochondrial genome maintenance and distribution, decreased respiratory metabolism, abnormal cell cycle progression, and changes in nitrogen and carbon source utilisation. Studies of related genes in other species connect the family to mitochondrial genome maintenance and petite-negative phenotypes.