NEO1 — Verticillium dahliae

Summary

VDAG_08403 (VdNEO1) on chromosome DS572714 of Verticillium dahliae encodes a P-type ATPase family protein with lysophosphatidylserine, glycoceramide, phosphatidylcholine, phosphatidylethanolamine, and phosphatidylserine flippase and floppase activities, as well as P-type calcium transporter activity, ATPase-coupled intramembrane lipid carrier activity, and calcium ion binding. The gene is associated with phospholipid and ceramide translocation, endocytic recycling, post-Golgi transport, sporulation, secretion, endocytosis, and establishment of cell polarity. It localizes to the trans-Golgi network membrane, endosome membrane, division septum, and fungal-type vacuole membrane. Associations include abnormal pseudohyphal growth, decreased cell death, increased ionic stress and UV resistance, decreased cold sensitivity, abnormal chitin deposition and septum formation, and altered cell cycle progression through G1 phase. Studies of related genes in other species link the family to reduced virulence.