APS1 — Verticillium dahliae

Summary

VDAG_08547 (symbol APS1, also known as VdVAS2 or VAS2) in Verticillium dahliae encodes a protein with clathrin adaptor activity and clathrin binding, belonging to the AP-1/sigma family and containing a longin-like domain superfamily. The gene is associated with multiple vesicle-mediated transport processes, including endosome-to-plasma membrane protein transport, clathrin-mediated endocytosis, Golgi-to-vacuole transport, retrograde endosome-to-Golgi transport, and vacuolar protein sorting. It localises to the AP-3 adaptor complex, cytoplasmic vesicle membrane, endosome, Golgi complex, cytosol, and nucleus. Phenotypic associations include decreased protein transport, abnormal vacuolar transport, decreased nutrient uptake, increased vegetative growth rate, abnormal cell wall morphology, abnormal sporulation, and decreased ionic stress resistance. Studies of related genes in other species link the family to loss of pathogenicity and reduced virulence.