RAD1 — Neurospora crassa

Summary

NCU07440 (RAD1, also known as mus-38) on Neurospora crassa chromosome 1 encodes a protein of the XPF nuclease family, possessing 3' overhang single-stranded DNA endonuclease activity, single-stranded DNA endonuclease activity, and damaged DNA binding. The protein localises to the nucleus, cytoplasm, mitotic spindle pole body and the NEF1 complex. RAD1 is associated with multiple DNA repair pathways including nucleotide excision repair (NER), interstrand crosslink repair, homologous recombination, and double-strand break repair via single-strand annealing, as well as meiotic recombination, mating-type switching, and resolution of meiotic recombination intermediates. Phenotypically, RAD1 is linked to absent X-ray resistance, increased necrotic cell death, decreased lifespan, abnormal sporulation, delayed exponential growth, premature chromosome segregation, arrested meiosis, and altered cell cycle progression through G2/M phase. Studies of related genes in other species link the family to cell membrane stability and oxidative stress response.