PBS2 — Neurospora crassa

Summary

NCU00587 (PBS2, also known as os-5) on Neurospora crassa chromosome 1 encodes a dual-specificity mitogen-activated protein kinase kinase (MAPKK) with MAP kinase scaffold activity, classified under EC 2.7.12.2. The product belongs to the Ser/Thr kinase family and possesses protein serine/threonine kinase, protein tyrosine kinase, and ATP-binding activities. PBS2 is associated with the osmosensory signaling pathway via the Sho1 osmosensor, the cellular HOG response, p38 cascade, cell integrity MAPK cascade, pheromone response, and filamentous growth. It is linked to regulation of ascospore formation, appressorium formation, cell wall organization, calcium-mediated signaling, and actin filament organization. The protein localizes to the cytoplasm, cell tip, bud neck, division septum, and mating projection tip. Phenotypic associations include increased acquired thermotolerance, altered sporulation and mating efficiency, abnormal carbon source utilization, increased fermentative and anaerobic growth, and absence of desiccation and hyperosmotic stress resistance.