CLM2 — Magnaporthe oryzae 70-15

Summary

MoCLM2 (MGG_07488) on chromosome 3 of Magnaporthe oryzae 70-15 encodes a cytochrome P450 with heme and iron binding, oxidoreductase activity acting on paired donors with oxygen incorporation, and 25-hydroxyvitamin D-1 alpha hydroxylase activity. The gene is associated with demethylation, mitophagy, sporulation, hyphal growth, and ergosterol anabolism, and localizes to membranes. Phenotypic associations include decreased micronucleophagy, nucleophagy, and ribophagy; absent hyperosmotic stress resistance and cytokinesis; abnormal plasma membrane morphology, cell cycle progression through the G2/M phase transition, and entry into G0; increased cell death and lifespan; and arrested vegetative and exponential growth. The gene is also linked to decreased invasive growth, nutrient uptake, vacuolar transport, and utilization of iron and phosphorus sources, alongside increased fermentative metabolism and carbon source utilization. Studies of related genes in other species connect the family to sterol biosynthesis and P450 function.