PTR1 — Fusarium oxysporum f. sp. lycopersici 4287

Summary

FOXG_00950 (FoPTR1, also known as FoTOM1) on chromosome 1 of Fusarium oxysporum f. sp. lycopersici 4287 encodes a HECT-type E3 ubiquitin ligase with ubiquitin-protein transferase activity and phospholipid binding capacity. The gene is associated with protein polyubiquitination, ubiquitin-dependent proteolysis, and multiple vacuolar transport processes including cytoplasm-to-vacuole targeting via the NVT pathway and protein localization to the vacuole. FoPTR1 is linked to negative regulation of L-leucine import, Golgi-to-plasma membrane protein transport, and uracil import. Phenotypic associations include abnormal cell cycle progression (G1/S, G2/M, metaphase), delayed chromosome segregation, increased sporulation, increased fermentative growth rate, abnormal mitochondrial transport and nuclear export, increased freeze-thaw resistance, and altered entry into stationary phase. The protein localizes to the cytoplasm, nucleus, Golgi, membrane, cell periphery, and nucleolus. Related genes in other species link the family to reduced virulence and oxidative stress response.