CYS3 — Neurospora crassa

Summary

NCU09230 (CYS3) of Neurospora crassa encodes a pyridoxal phosphate-dependent enzyme with cystathionine gamma-lyase, O-acetylhomoserine (thiol)-lyase, homocysteine synthase, cysteine synthase and cysteine-S-conjugate beta-lyase activities (EC 4.4.1.1). The gene is associated with transsulfuration, L-homocysteine and L-methionine biosynthesis, cysteine anabolism, sulfur metabolism, alkaloid metabolism, sulphate transport, and cell growth. The encoded protein localises to the cytoplasm, cytosol, nucleus and peroxisome. Phenotypic associations link CYS3 to abnormal nutrient utilisation and sulfur source utilisation, increased X-ray and UV resistance, increased aneuploidy, altered cell cycle progression (G2/M and G1/S checkpoints), decreased prion inheritance, absent filamentous growth, and abnormal nucleophagy and mitophagy. Studies of related genes in other species connect the family to O-acetylhomoserine aminocarboxypropyltransferase activity (EC 2.5.1.49) and oxidative stress response.