PGI1 — Neurospora crassa

Summary

NCU07281 (PGI1, gpi-1) on Neurospora crassa chromosome 4 encodes a glucose-6-phosphate isomerase, confirmed by its glucose-6-phosphate isomerase activity and membership in the phosphoglucose isomerase family. The gene is associated with canonical glycolysis, gluconeogenesis, the pentose-phosphate shunt, and glucose-6-phosphate metabolism. PGI1 is linked to diverse phenotypes including abnormal or absent flocculation and biofilm formation, delayed carbon source utilization, absent anaerobic growth, increased fermentative growth and metabolism, altered adhesion, absent innate thermotolerance, and abnormal prion inheritance and formation. The protein localizes to the cytosol, mitochondria, and the fungal biofilm matrix. Studies of related genes in other species connect the family to loss of pathogenicity and reduced virulence, as well as abnormal cytokinesis and cell cycle progression.