CLR4 — Neurospora crassa

Summary

NCU04402 (CLR4, dim-5) on Neurospora crassa chromosome 4 encodes a histone-lysine N-methyltransferase (HKMT) with a SET domain and pre-SET and post-SET domains, classified as EC 2.1.1.355. The gene product exhibits histone H3K9 methylase, monomethylase, dimethylase and trimethylase activities, as well as histone H3K36 and H3K4 methylase activities, and is associated with reader activity of ubiquitin-modified histones and H3K9me2/3 marks. CLR4 is associated with heterochromatin island assembly, RNAi-mediated heterochromatin assembly at centromere, RNAi-mediated co-transcriptional gene silencing (CTGS), suppression of meiotic recombination, X chromosome inactivation, circadian rhythm, DNA replication licensing, DNA-templated transcription elongation, and nuclear transport. The protein localises to the CLRC complex, Set1C, chromatin, nucleus, cytosol, spindle pole body, and chromosome. Studies of related genes in other species link the family to additional histone methylation activities.