DBP2 — Neurospora crassa

Summary

NcDBP2 (NCU02803) on Neurospora crassa chromosome 1 encodes a DEAD-box RNA helicase of the PRP28/DDX23-like family, with ATP hydrolase, RNA helicase, nucleic acid and mRNA binding activities, and first spliceosomal transesterification activity (EC 3.6.4.13). The gene is associated with mRNA splicing via the spliceosome, mRNA 5'-splice site recognition, ribosome biogenesis, and fruiting body formation. It localises to the U5 snRNP, catalytic step 2 spliceosome, nucleus and cytoplasm. Phenotypic associations include abnormal mitotic recombination, decreased mating type switching, absent sporulation efficiency, increased thermotolerance and gamma ray resistance, delayed vegetative and exponential growth, increased fermentative growth and carbon source utilisation, and altered cell cycle progression including increased S phase rate, premature G1/S transition, and delayed or arrested anaphase. Related genes in other species are linked to oxidative stress response and unaffected pathogenicity.