THR1 — Magnaporthe oryzae 70-15

Summary

MGG_09550 (MoTHR1, THR1) on chromosome 3 of Magnaporthe oryzae 70-15 encodes a homoserine kinase (EC 2.7.1.39) with GHMP kinase domains and ATP-binding activity. The gene is associated with threonine anabolism, methionine metabolism, spore germination, and melanin, sterol, and aflatoxin anabolism. The encoded protein localises to the cytoplasm and cytosol. Phenotypic associations include altered cell cycle progression (S-phase arrest, G2/M delay, M-phase arrest), increased UV and X-ray resistance, decreased cold sensitivity, increased pseudohyphal growth, abnormal endocytosis, vacuolar and peroxisomal transport, decreased nutrient and iron utilisation, and increased carbon source utilisation. Studies of related genes in other species link the family to petite-negative growth and altered resistance to UV, X-rays, and enzymatic treatment.