THI-4 — Verticillium dahliae

Summary

VDAG_05690, symbol THI-4 and also known as VdTHI-4, encodes a phosphomethylpyrimidine kinase (THI20) on chromosome DS572705 of Verticillium dahliae. The product possesses thiaminase, phosphomethylpyrimidine kinase, and hydroxymethylpyrimidine kinase activities, binds ATP, and belongs to the HMP/HMP-P kinase and thiaminase-2/PQQC domain families. It is associated with thiamine (TPP) biosynthesis and DNA repair, and localises to the cytoplasm, cytosol, and nucleus. The gene is linked to vascular wilt disease in Nicotiana benthamiana. Phenotypic associations include altered nucleolus size, replicative lifespan, competitive fitness, vacuolar morphology, and chemical resistance. Studies of related genes in other species connect the family to oxidative stress resistance and toxin resistance.