RPB2 — Verticillium dahliae

Summary

VdRPB2 (VDAG_05625) in Verticillium dahliae encodes a protein with DNA-directed RNA polymerase activity (EC 2.7.7.6), RNA-directed RNA polymerase activity, and ribonucleoside, RNA, DNA, and zinc ion binding. The protein localizes to the nucleus, cytosol, chromatin, and centric heterochromatin, and is a component of RNA polymerase I, II, and III complexes. It is associated with transcription initiation, elongation, and termination from all three polymerase promoters, snRNA transcription by RNA polymerase III, and RNAi-mediated heterochromatin assembly at centromeres. Phenotypic associations link the gene to abnormal cell cycle progression, meiosis, chromosome segregation, and increased aneuploidy, as well as decreased radiation resistance, altered fermentative and respiratory metabolism, and abnormal spore wall formation, shmoo formation, and endocytosis.