BRN1 — Verticillium dahliae

Summary

VDAG_04141, also known as VdBRN1 or VdCND2, encodes a condensin barren subunit 2 family protein in Verticillium dahliae. The product possesses dsDNA, ssDNA, and chromatin binding activities, as well as ATP hydrolase activity. VdBRN1 is associated with mitotic chromosome condensation, attachment of spindle microtubules to chromosomes, DNA repair, cell division, conidia formation, and melanin anabolism. It localizes to the condensin complex, CENP-A-containing chromatin, rDNA heterochromatin, heterochromatin, chromatin, cytoplasm, cytosol, and nucleus. The gene has been linked to numerous abnormal phenotypes including altered colony size, cell cycle progression defects (delayed G1/S transition, premature anaphase, delayed metaphase-anaphase transition, mitotic arrest), increased thermotolerance and gamma ray resistance, delayed vegetative growth, absent cell fusion and spore germination, abnormal shmoo formation and bipolar budding, increased nucleus size, and decreased mitotic recombination. Studies of related genes in other species connect the family to chromosome segregation and cell cycle regulation.