TPS3 — Lactuca sativa

Summary

LsTPS3 (TPS3) on chromosome 2 of garden lettuce encodes a terpene synthase, possessing multiple terpene cyclase and synthase domains and exhibiting a broad range of enzyme activities including isoprene, alpha-farnesene, 1,8-cineole, tricyclene, R-linalool, (R)-limonene, (4S)-limonene, pinene, sabinene, (E)-beta-ocimene, myrcene, sesquiterpene, and general terpene synthase activities, as well as lyase activity. The gene is associated with the biosynthesis of numerous terpenoids, including monoterpenes, sesquiterpenes, and diterpenoids, and with the catabolism of farnesyl diphosphate and metabolism of geranyl diphosphate. LsTPS3 is associated with responses to herbivores and insects, green leaf volatile biosynthesis, circadian rhythm, and response to jasmonic acid. It is linked to traits such as nitrate uptake, leaf composition, seedling growth and development, and organ identity, as well as phenotypes including herbivore defense, volatile terpene emission, and carbon allocation. The encoded protein localizes to the chloroplast stroma, cytosol, mitochondria, Golgi complex, and plant-type vacuole.