CRH1 — Fusarium oxysporum f. sp. lycopersici 4287

Summary

FoCRH1 (FOXG_11947) on chromosome 13 of Fusarium oxysporum f. sp. lycopersici 4287 encodes a GH16 family transglycosidase with IgE-binding activity, belonging to the Glyco_hydro_16_CRH1_predicted domain family. The gene is associated with chitin and carbohydrate metabolism, cell wall organization, and pH reduction. The product localizes to the fungal-type cell wall, hyphal and yeast-form cell walls, host cell cytoplasm, extracellular vesicles, vacuoles, and cellular bud sites. Phenotypic associations include abnormal cell wall morphology, increased chitin deposition, altered cell death, abnormal spore wall formation, increased biofilm formation, decreased invasive growth, and abnormal vegetative growth, budding, and cell shape. The gene is linked to expression during tomato infection at 2, 4, and 6 days post-inoculation. Studies of related genes in other species connect the family to reduced virulence and stress resistance.