CAF — Nicotiana attenuata

Summary

A4A49_02244 (CAF/DCL1) on coyote tobacco chromosome 8 encodes a Dicer-like protein with RNase III, helicase, PAZ and dsRNA-binding domains, annotated with ribonuclease III, DNA glycosylase, AP lyase, RNA methyltransferase and helicase activities. It is associated with pri-miRNA and pre-miRNA processing, siRNA processing, gene silencing by miRNA-directed mRNA cleavage, and regulation of adaxial/abaxial pattern formation, seed maturation, meristem development and cell fate specification. The gene is linked to traits including leaf, floral organ and inflorescence morphology, vegetative vigor, seed dormancy, root mass density, calcium concentration, herbicide sensitivity, osmotic and light responses, and biotic and abiotic stress responses. Phenotypic associations include herbivore resistance, jasmonate-isoleucine accumulation, nicotine accumulation, antiviral defense, systemic RNA interference, and resistance to Fusarium brachygibbosum and Sclerotinia sclerotiorum.