ISA — Triticum turgidum subsp. durum

Summary

TRITD7BV1G064650 (symbol ISA, also known as TtISA1 or PHS8) on durum wheat chromosome 7B encodes a glycosyl hydrolase family 13 catalytic domain-containing protein with isoamylase activity (EC 3.2.1.68) and 1,4-alpha-glucan branching enzyme activity. The gene is associated with amyloplast organization, starch and amylopectin anabolism and breakdown, glycogen metabolism, and embryo development ending in seed dormancy. It localizes to the amyloplast, cytoplasm, and isoamylase complex. The gene is linked to pathways for amylopectin synthesis, seed development, root gravitropism, and responses to abiotic stimuli. Associations include polysaccharide content, starch granule biosynthesis, starch synthesis and structure, and phytoglycogen accumulation. Studies of related genes in other species connect the family to starch metabolism, endosperm traits, grain appearance and texture, amylose content, seed weight, and seedling vigor.

Annotations

Chromosome
7B
Related genes
ISA, ISA1
GO terms
carbohydrate metabolism, Embryo Development Ending In Seed Dormancy, glycogen anabolism, Amyloplast, starch anabolism, 1,4-alpha-glucan Branching Enzyme Activity, Cation Binding, GO:0009507
Publications
15749471, 23393426, 25663508, PMID:15862090, PMID:7773016, PMID:12834068, PMID:16953433, PMID:20018713
Traits
arabinose metabolism, Modified amylopectin structure with increased short chains, decreased long chains, Reduced Starch, Increased Soluble Glucan Accumulation, Reduced Leaf Starch Content, Defective Amylopectin Biosynthesis, Reduced Starch, Phytoglycogen Production, No Change in Amylose-Amylopectin Ratio, Normal Maltose and Maltotriose Production, Increased water soluble polysaccharides content