ISA — Oryza sativa Indica Group

Summary

BGIOSGA026650 (ISA, also known as PHS8, ISA1, OiISA1, or OiPHS8) on chromosome 8 of Indica rice encodes a protein with isoamylase activity (EC 3.2.1.68), 1,4-alpha-glucan branching enzyme activity, cation binding, and O-glucosyl hydrolase activity, belonging to the glycoside hydrolase family 13. This gene is associated with starch metabolism, including amylopectin anabolism and breakdown, glycogen metabolism, and regulation of starch metabolism. It is linked to amyloplast organization, seed and embryo development, response to salt and salinity, and protein phosphorylation. The encoded protein localizes to the isoamylase complex, chloroplast isoamylase complex, and amyloplast. ISA is associated with starch and endosperm phenotypes, sugar content, starch granule biosynthesis, starch synthesis and structure, and phytoglycogen accumulation. Studies of related genes in other species link the family to starch metabolism, starch biosynthesis and accumulation, and traits including sugary endosperm, amylose content, grain appearance and texture, seed weight, and seedling vigor.