TPS3 — Lactuca sativa

Summary

LsTPS3 (gene symbol TPS3, chromosome 8) encodes a terpene synthase with multiple annotated activities including alpha-farnesene, 1,8-cineole, tricyclene, R-linalool, (R)-limonene, (4S)-limonene, pinene, sabinene, (E)-beta-ocimene, myrcene, sesquiterpene, and general terpene synthase activities, along with lyase activity and magnesium binding. The protein contains terpene cyclase and synthase domains. LsTPS3 is associated with monoterpenoid and sesquiterpenoid biosynthesis, geranyl diphosphate metabolism, and responses to herbivores, insects, wounding, and jasmonic acid, as well as circadian rhythm and green leaf volatile biosynthesis. It has been linked to nitrate uptake, leaf composition, seedling growth, organ identity, herbivore defense, volatile terpene emission, carbon allocation to organic acids and starch, and leaf development. The protein localizes to chloroplast stroma, vacuole, Golgi, mitochondria, and cytosol. Studies of related genes in other species connect the family to wound response, volatile terpene production, and linalool biosynthesis.