ISA — Triticum turgidum subsp. durum
Summary
TRITD7AV1G095990 (symbol ISA, also known as TtISA1 or PHS8) on durum wheat chromosome 7A encodes an isoamylase, a glycosyl hydrolase family 13 catalytic domain-containing protein with isoamylase activity (EC 3.2.1.68) and 1,4-alpha-glucan branching enzyme activity. This gene is associated with amyloplast organization, starch and amylopectin anabolism, glycogen metabolism, and embryo development ending in seed dormancy. It is linked to the isoamylase complex and localizes to the amyloplast and cytoplasm. The gene has been connected to starch granule biosynthesis, starch synthesis and structure, and phytoglycogen accumulation. Studies of related genes in other species link the isoamylase family to starch metabolism, amylopectin structure, and traits including grain appearance, texture, color, amylose content, seed weight, and endosperm characteristics such as sugary, floury, and dull endosperm.
Annotations
- Chromosome
- 7A
- Related genes
- ISA, ISA1
- GO terms
- carbohydrate metabolism, Embryo Development Ending In Seed Dormancy, glycogen anabolism, Amyloplast, starch anabolism, 1,4-alpha-glucan Branching Enzyme Activity, Cation Binding, GO:0009507
- Publications
- 15749471, 23393426, 25663508, PMID:15862090, PMID:7773016, PMID:12834068, PMID:16953433, PMID:20018713
- Traits
- arabinose metabolism, Modified amylopectin structure with increased short chains, decreased long chains, Reduced Starch, Increased Soluble Glucan Accumulation, Reduced Leaf Starch Content, Defective Amylopectin Biosynthesis, Reduced Starch, Phytoglycogen Production, No Change in Amylose-Amylopectin Ratio, Normal Maltose and Maltotriose Production, Increased water soluble polysaccharides content