ISA3 — Triticum aestivum

Summary

TRAESCS5B02G246400 (symbol ISA3, TaISA3-5B) on bread wheat chromosome 5B encodes a protein with isoamylase activity and 1,4-alpha-glucan branching enzyme activity, belonging to the glycoside hydrolase superfamily. The gene is associated with starch metabolism, amylopectin anabolism, amyloplast organization, and sucrose transport, and its product localizes to the isoamylase complex, chloroplast starch grain, and amyloplast. Associations include starch content, grain filling period, net photosynthetic rate, stripe rust, and vascular bundle number. Phenotypic links connect ISA3 to starch granule biosynthesis and number, amylose content, leaf starch content, cold acclimation, and altered carbohydrate chain length distribution. Studies of related genes in other species link the family to starch degradation, amylopectin structure, and starch mobilization, and to grain yield, anthesis silking interval, and cold and drought tolerance.