F3H — Lactuca sativa

Summary

LsF3H (LSAT_V11C300129900) on lettuce chromosome 3 encodes a 2-oxoglutarate/Fe(II)-dependent dioxygenase with flavanone 3-dioxygenase, flavonol synthase and chalcone isomerase activities, and binds vitamin C and metals. The gene is associated with anthocyanin, flavonoid, coumarin and sucrose biosynthesis, pigment accumulation, photomorphogenesis, and responses to UV, UV-B, cold, auxin and fungal molecules. It localises to the tonoplast, ER, cytosol, cytoplasm and nucleus. Associations include leaf color, anthocyanin and flavonoid content, chlorophyll content, shoot fresh and dry weight, quantum yield, stomatal resistance, oxidative stress response and UV-B light sensitivity. Phenotypic links connect F3H to anthocyanin and proanthocyanidin accumulation, flavonoid synthesis, pollen traits, powdery mildew resistance, lateral root and root hair formation, cold acclimation, circadian clock regulation, photosynthetic activity and genome stability. Studies of related genes in other species link the family to seed coat pigmentation, UV-B tolerance and flavonol biosynthesis.