ISA — Hordeum vulgare subsp. vulgare
Summary
HORVU.MOREX.R3.7HG0681220, designated HvISA or HvISA1 on barley chromosome 7H, encodes an isoamylase (EC 3.2.1.68) with glycoside hydrolase family 13 domains and is also annotated for 1,4-alpha-glucan branching enzyme activity and cation binding. The gene is associated with starch granule initiation, amyloplast organization, amylopectin and starch anabolism, starch breakdown, glycogen metabolism, programmed cell death, and embryo development ending in seed dormancy. The isoamylase complex localizes to the chloroplast and amyloplast. HvISA has been linked to floury endosperm, seedling vigor, starch content, starch granule biosynthesis, starch synthesis and structure, and phytoglycogen accumulation. Studies of related genes in other species connect the family to starch metabolism, starch biosynthesis and accumulation, and endosperm starch traits.
Annotations
- Chromosome
- 7H
- Related genes
- ISA, ISA1
- GO terms
- NO storage, carbohydrate metabolism, Embryo Development Ending In Seed Dormancy, glycogen anabolism, Amyloplast, starch anabolism, 1,4-alpha-glucan Branching Enzyme Activity, Cation Binding
- Publications
- 15749471, 23393426, 25663508, PMID:15862090, PMID:7773016, PMID:12834068, PMID:16953433, PMID:20018713
- Traits
- arabinose metabolism, Modified amylopectin structure with increased short chains, decreased long chains, Reduced Starch, Increased Soluble Glucan Accumulation, Reduced Leaf Starch Content, Defective Amylopectin Biosynthesis, Reduced Starch, Phytoglycogen Production, No Change in Amylose-Amylopectin Ratio, Normal Maltose and Maltotriose Production, Increased water soluble polysaccharides content