BR6OX — Brassica oleracea var. botrytis

Summary

BoBR6OX, located on chromosome 7 of cauliflower (Brassica oleracea var. botrytis), encodes a cytochrome P450 family protein with brassinolide synthase, 22alpha-hydroxysteroid 23-monooxygenase, steroid 22S-hydroxylase, and fatty acid 2-hydroxylase activities. The gene is associated with brassinosteroid anabolism and homeostasis, sterol and cholesterol metabolism, steroidogenesis, and saponin anabolism. It is linked to leaf, stamen, and petal development, megagametogenesis, skotomorphogenesis, microtubule bundling, and response to light stimulus. The encoded protein localizes to the cytoplasmic vesicle membrane, endoplasmic reticulum, membrane, and mitochondria. Associations with stem diameter, shoot fresh weight, plant height, pedicel elongation, drought tolerance, abscisic acid responsiveness, and brassinolide accumulation under boron deficiency have been observed. Studies of related genes in other species link the family to brassinosteroid biosynthesis, hypocotyl elongation, vegetative development, grain yield, and grain quality.