DCL2 — Brassica oleracea var. botrytis

Summary

BoDCL2, encoded by gene BOLND125_C05P072380.1 on cauliflower chromosome 5, is a Dicer-like protein containing RNase III, PAZ, helicase, and dsRNA-binding domains. It has ribonuclease III and helicase activities, binds RNA, ATP, metals, and proteins, and localises to both the cytoplasm and nucleus. The gene is associated with RNA VIGS, hypermethylation of CpG island, ta-siRNA processing, miRNA processing, siRNA processing, maintenance of shoot apical meristem identity, polarity specification of adaxial/abaxial axis, PTGS, reproductive structure development, and antiviral response. It is also associated with antiviral defense, viral siRNA biogenesis, plant development, transitive silencing, RNA silencing, viral resistance, stress tolerance, antiviral immunity, development, virus resistance, systemic RNA interference, phloem transport, viral siRNA production, antiviral resistance, transgenerational stress memory, root microbiota composition, miRNA biogenesis, ABA sensitivity, and siRNA production. Related genes in other species link the family to systemic antiviral silencing, egg cell development, flowering time, and transgenerational insect resistance.