P5CS2 — Brassica oleracea var. botrytis

Summary

BoP5CS2, located on chromosome 4 of cauliflower (Brassica oleracea var. botrytis), encodes a protein with glutamate 5-kinase activity and glutamylphosphate reductase activity, and belongs to the glutamate-5-kinase and aldehyde dehydrogenase domain families. The gene is associated with L-proline biosynthetic process, proline transport, hyperosmotic salinity response, microsporogenesis, seed germination, embryo development ending in seed dormancy, and response to abscisic acid. It is linked to proline content, pollen fertility, freezing tolerance, salt tolerance, vegetative development, root meristem size, sodium ion accumulation, and embryo lethal, proline-rescuable with cotyledon defects. The encoded protein localises to the cytoplasm, mitochondria, and plasmodesmata. Studies of related genes in other species connect the family to proline biosynthesis, abiotic stress tolerance, root elongation, flowering time, and pathogen resistance.