BR6OX — Brassica oleracea var. botrytis

Summary

BoBR6OX, located on chromosome 2 of cauliflower (Brassica oleracea var. botrytis), encodes a cytochrome P450 family protein with brassinolide synthase activity, a monooxygenase that acts on paired donors incorporating molecular oxygen. This gene is associated with brassinosteroid homeostasis and anabolism, sterol and cholesterol metabolism, steroidogenesis, and protein farnesylation. It is linked to petal, stamen, leaf, and gynoecium development, megagametogenesis, skotomorphogenesis, and response to light stimulus. The encoded protein localises to the cytoplasmic vesicle membrane, endoplasmic reticulum, and mitochondria. BoBR6OX is associated with stem diameter, shoot fresh weight, plant height, pedicel elongation, and drought tolerance, as well as brassinolide accumulation under boron deficiency and abscisic acid responsiveness. Related genes in other species link the family to grain yield and quality, seed dormancy, and root morphology.