REF2 — Brassica oleracea

Summary

BoREF2 (BOLC102_C04P044580.1) on cabbage chromosome 4 encodes a cytochrome P450 with costunolide synthase activity, heme and iron binding, and oxidoreductase activity acting on paired donors with NAD(P)H. The gene is associated with glucosinolate, coumarin, sesquiterpene, terpenoid, alkaloid, phenylpropanoid, and flavonoid biosynthesis, as well as very-long-chain fatty acid metabolism, response to UV and insects, fruit ripening, leaf development, and downregulation of programmed cell death. It localises to the endoplasmic reticulum, nuclear envelope, membrane, cytosol, and plasmalemma. Associations include stem thickness, leaf width, sugar content, water, high-temperature, and low-temperature stress responses, leaf senescence, and disease tolerance. Related genes in other species link the family to aliphatic glucosinolate biosynthesis, powdery mildew resistance, and defence against biotrophic pathogens.