F5H — Brassica napus

Summary

BnF5H (DARMORV10_C07P61600.1) on chromosome C07 of oilseed rape (Brassica napus) encodes a cytochrome P450 family 84A protein with ferulate 5-hydroxylase activity, also annotated as a monooxygenase and oxidoreductase that binds iron and heme. The gene is associated with lignin anabolism, phenylpropanoid metabolism, cell wall modification, and the downregulation of chlorophyll anabolism. It is linked to very-long-chain fatty acid, cutin, alkane, and terpenoid anabolism, as well as sesquiterpene biosynthesis, fruit ripening, defense response, and UV-B response. The encoded protein localizes to the endoplasmic reticulum membrane and plasma membrane. Associations with stem strength, drought tolerance, root morphology, lateral root development, plant growth, lignin composition, syringyl lignin biosynthesis, cell wall digestibility, saccharification efficiency, sugar release, sinapine content, and disease resistance have been reported. Related genes in other species are linked to biomass yield, male sterility, and lignin content.