F3H — Brassica juncea

Summary

BJUB08G08960S (BjF3H) on chromosome B08 of Indian mustard encodes a 2-oxoglutarate-dependent dioxygenase with flavanone 3-dioxygenase, flavonol synthase and chalcone isomerase activities, belonging to the DIOX_N and IPNS-like Fe2OG oxygenase domain families. The gene is associated with anthocyanin, flavonoid, coumarin and phenylpropanoid biosynthesis, pigmentation, responses to UV-B, UV light, auxin and fungal molecules, and nitric oxide storage. It localises to the cytosol, nucleus, endoplasmic reticulum and tonoplast. Associations include seed and leaf color, stamen morphology, root phototropism, anthocyanin content, oxidative stress response, respiration rate, and UV-B sensitivity. Phenotypic links connect BjF3H to proanthocyanidin and flavonoid accumulation, seed coat pigmentation, cold acclimation, circadian clock regulation, photosynthetic activity, lateral root development, genome stability, and resistance to cyst and root-knot nematodes. Studies of related genes in other species link the family to seed coat pigmentation, UV-B tolerance and flavonoid production.