GSH2 — Brassica juncea

Summary

BJUB02G67580S (GSH2) on chromosome B02 of Indian mustard (Brassica juncea) encodes a glutathione synthase, a homodimeric enzyme that catalyzes the final step of glutathione biosynthesis. The protein contains characteristic glutathione synthase domains and exhibits glutathione synthase activity, glutathione binding, ATP binding, magnesium binding, and protein homodimerization activity. It is localized to the cytosol. The gene is associated with glutathione anabolism, sulfate assimilation, sulfur metabolism, and general homeostasis. It has been linked to the gamma-glutamyl cycle, cofactor biosyntheses, and broader metabolism and regulation pathways. Related genes in other species link the family to glutathione biosynthesis (EC 6.3.2.3), supporting the conserved role of this gene in cellular redox balance and sulfur assimilation.